Comparison Effect of Leaves and Bark Extract of Eucalyptus (Melaleuca leucadendra), Sappan (Caesalpinia sappan), and Cinnamon (Cinnamomum zeylanicum) to Reduce Streptococcus mutans Biofilm Formation
نویسندگان
چکیده
To evaluate the effect of eucalyptus, sappan, and cinnamon leaf bark extract on percentage S. mutans biofilm formation. The test group was divided into a negative control (1% Dimethyl sulfoxide), treatment (eucalyptus, leaves at concentrations 50%, 25%, 12.5%, respectively), positive (0.2% chlorhexidine). Each concentration barks extract, Brain Heart Infusion Broth (BHI-B), bacteria according to McFarland 0.5 standard, control, were added 96-well microplate. They incubated 37 °C for twenty-four hours before being rinsed with phosphate buffer saline (PBS) stained 0.1% crystal violet. optical density then measured using microplate reader 540 nm wavelength. absorbance value is factored bacterial adhesion inhibition formula. A statistical revealed significant difference (p < 0.05) in between three extracts DMSO). There all except 50% sappan group. exhibit no > control. Eucalyptus, demonstrated effectiveness extractant inhibiting formation masses by comparison other
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ژورنال
عنوان ژورنال: Majalah kedokteran gigi Indonesia
سال: 2023
ISSN: ['2460-0164', '2442-2576']
DOI: https://doi.org/10.22146/majkedgiind.83283